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KMID : 0363020070370040655
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Journal of Korean Academy of Periodontology
2007 Volume.37 No. 4 p.655 ~ p.669
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Effects of H2O2and ascorbic acid on TIMP-2, Type 1 collagen, and PDLs22 Levels in human periodontal Ligament fibroblasts
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Choi Yong-Sun
Kim Byung-Wook
Jang Hyun-Seon
Choi Sung-Mi
Kim So-Young
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Abstract
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Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases. And vitamin C has shown a protective effect for the tissues. The aim of this study was to evaluate the effects of and ascorbic acid on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase (TIMP: TIMP-1, TIMP-2), Type 1 collagen, fibronectin, and PDLs22 level in human periodontal ligament fibroblasts (hPDLF) via reverse transcription-polymerase chain reaction (RT-PCR). hPDLF was obtained from a healthy periodontium and cultured in Dulbecco’s modified Eagles’s medium plus 10% fetal bone serum. The concentration of ascorbic acid in hPDLF was , and that of in hPDLF was 0.03% and 0.00003%. Ascorbic acid only, only and mixture of ascorbic acid and were applied with hPDLF for 1-, 3-, and 30-min. respectively. The gene expression of MMP-1-, TIMP-1-, TIMP-2-, Type 1 collagen-, fibronectin-, and PDLs22-mRNA in hPDLF was analysed via RT-PCR. The results were as follows; 1. hPDLF in response to 30-min. incubation with 0.03% did not show any gene expression. 2. In all the experimental groups, the gene expression of fibronectin mRNA showed the decreased tendency compared to control. 3. In all the experimental groups, the gene expression of TIMP-1 mRNA showed the tendency similar to control. 4. hPDLF in response to 30-min. incubation with 0.03% and ascorbic acid increased mRNA induction for MMP-1. 5. In all the experimental groups, hPDLF increased mRNA induction for PDLs22, collagen type 1, and TIMP-2 compared to control. Within the limited experiments, and ascorbic acid increased mRNA induction for PDLs22, collagen type 1, TIMP-2 in hPDLF. More research will be needed in order to confirm the relative importance of the different roles of ROS and antioxidants in hPDLF from a periodontal regeneration or repair standpoint.
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KEYWORD
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H2O2, ascorbic acid, human Periodontal Ligament fibroblasts
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